Description
Data-Driven qRT-PCR Normalization Using NORMAgene.
Description
Enables correction for technical variance in raw quantitative reverse transcription polymerase chain reaction (qRT-PCR) data using the least squares-based NORMAgene data-driven normalization algorithm originally described by Heckmann et al. (2011) <doi:10.1186/1471-2105-12-250>. Performs normalization of raw crossing threshold values (CT) and also calculates relative variability metrics that can be used to assess the impact of normalization on variance.