Description
Real-Time PCR Data Sets by Boggy et al.
Description
Real-time quantitative polymerase chain reaction (qPCR) data sets by Boggy et al. (2008) <doi:10.1371/journal.pone.0012355>. This package provides a dilution series for one PCR target: a random sequence that minimizes secondary structure and off-target primer binding. The data set is a six-point, ten-fold dilution series. For each concentration there are two replicates. Each amplification curve is 40 cycles long. Original raw data file: <https://journals.plos.org/plosone/article/file?type=supplementary&id=10.1371/journal.pone.0012355.s004>.
README.md
boggy
{boggy} provides the real-time PCR data set s1 by Boggy et al. (2010) in tidy format.
Installation
install.packages("boggy")
Usage
library(boggy)
library(ggplot2)
s1 |>
ggplot(mapping = aes(
x = cycle,
y = fluor,
group = well,
col = format(copies, big.mark = ",", scientific = FALSE)
)) +
geom_line(linewidth = 0.2) +
geom_point(size = 0.2) +
labs(color = "Copy number")
References
Gregory J. Boggy & Peter J. Woolf. A Mechanistic Model of PCR for Accurate Quantification of Quantitative PCR Data. PLoS ONE 5:8 (2010). doi: 10.1371/journal.pone.0012355.