Methods for Image-Based Cell Profiling.

cytominer

Typical morphological profiling datasets have millions of cells and hundreds of features per cell. When working with this data, you must

• clean the data

• normalize the features so that they are comparable across experiments

• transform the features so that their distributions are well-behaved ( i.e., bring them in line with assumptions we want to make about their disributions)

• select features based on their quality

• aggregate the single-cell data, if needed

The cytominer package makes these steps fast and easy.

Installation

You can install cytominer from CRAN:

install.packages("cytominer")

Or, install the development version from GitHub:

# install.packages("devtools")
devtools::install_github("cytomining/cytominer", dependencies = TRUE, build_vignettes = TRUE)

Occasionally, the Suggests dependencies may not get installed, depending on your system, so you'd need to install those explicitly.

Example

See vignette("cytominer-pipeline") for basic example of using cytominer to analyze a morphological profiling dataset.